Using CRISPR-Cas9, mutation W273L (position 274 in mice) was introduced into the GLB1 gene to generate a Morquio B model, while for GM1-gangliosidosis, a 20bp mutation was generated to remove the catalytic nucleophile of β-gal (β-gal-/-). The gene discussed is GLB1; the disease is GM1 gangliosidosis.