In fact, there were three cases with mutations in both KRAS and PIK3CA among our panel (CRC-017T: KRAS G13D, PIK3A Q546K, TP53 R81X and P27R; CRC-021T: KRAS G13D); however, they could not be used for in vivo validation due to the difficulty in obtaining sufficient PDX cells for in vivo combination efficacy test. Here, TP53 is linked to colorectal carcinoma.