Figure 4B shows that all types of cancer cells secreted VEGF upon treatment with different drugs, although its level was not always significantly increased in comparison with nontreated cells. IL-8 levels were significantly increased upon treatment with all drugs, with the exception of 5-FU and OX treatment of MDA-MB-231 cells and OX treatment of HCT116 cells (Figure 4A). This evidence concerns the gene CXCL8 and cancer.