Anti-tTG IgG contribution to tTG-LFRET signal in samples with low concentration of anti-tTG IgA could also explain the enhanced sensitivity of tTG-LFRET for CD diagnosis without IgG depletion (87.8% vs 77.0% with IgG depletion, Figs 1 and 2). Here, TGM2 is linked to Cowden disease.