We also used an alternative approach to rescue virus infection in TNK2 KO1 cells by reverting the 28 bp deletion in clone TNK2 KO1 using CRISPR-Cas9 with oligonucleotide-mediated homologous template-directed recombination (HDR); three synonymous mutations were included in the template to unambiguously identify a successful repair event (Figure 1—figure supplement 4H). This evidence concerns the gene TNK2 and viral infectious disease.