Flow cytometric analysis showed that α-CSPG4 rIgE was able to bind the human CSPG4-expressing melanoma cell line A2058 in a dose-dependent manner and similarly to its human counterpart, α-CSPG4 hIgE31 (Figure 2C), with 50% of the maximal mean fluorescence intensity reached by the two antibodies at similar concentration (1.57 μg/ml α-CSPG4 rIgE and 1.11 μg/ml α-CSPG4 hIgE). The gene discussed is CSPG4; the disease is melanoma.