Studies showed that HMGB1 was expressed in lung macrophages of COPD patients.25 To confirm these findings and to further evaluate the intracellular localization of HMGB1 in COPD patients, smokers and non‐smokers, we performed immunohistochemistry and immunofluorescence in lung tissues from these subjects undergoing lung resection for indicated diseases. This evidence concerns the gene HMGB1 and chronic obstructive pulmonary disease.