Another HTVI-based study modeling the consequences of RAS-driven carcinogenesis was later described by Zhang et al. Here, hydrodynamic injection of NRAS (G12V) and a constitutively active form of AKT (myrAKT) also yielded HCC and iCCA in mice 3 to 4 weeks after injection [14]. The gene discussed is PRRT2; the disease is hepatocellular carcinoma.