Co-expression of oncogenic Myc and NrasG12V, or Myc and AKT1 gave rise to multifocal liver carcinomas with HCC histology when introduced using HTVI, whereas, upon liver electroporation, the same combination of oncogenes resulted in focal iCCA or combined iCCA–HCC development. The gene discussed is PRRT2; the disease is hepatocellular carcinoma.