To next test whether human pancreatic cancer cell CM-induced myotube atrophy, which requires active IL-8, can be attenuated with inhibition of ERK1/2 activation, similar to IL-8 induced atrophy, we treated myotubes with PPC CM or L3.6pl CM, with or without U0126, for 48 h. Here, MAPK3 is linked to pancreatic neoplasm.