A previous study demonstrated the association between EMAST and the loss of MSH3 nuclear expression in CRC.20, 51 Further, an in vitro study provided the evidence that MSH3 knockdown could increase dinucleotide or tetranucleotide instability.23 However, our germline analysis demonstrated that only 4.5% (5/110) of EMAST patients had MSH3 germline mutations, all of which were missense variants. The gene discussed is MSH3; the disease is colorectal carcinoma.