Unlike the full-length TDP-43 protein, which localized only in the nucleus as expected, we confirmed that the truncated 35 kDa and 25 kDa TDP-43 proteins formed aggregates with a prevalent cytoplasmic localization (Figure 2a), mimicking the pathological aggregation observed in ALS/FTLD brains [28]. Here, TARDBP is linked to amyotrophic lateral sclerosis.