In our study, ANLN expression was strongly correlated with cell proliferation markers including CDK1, RACGAP1, MKI67, and FOXM1. In urothelial carcinoma cell lines, these genes showed a consistently strong positive correlation with the expression of ANLN, which is highly expressed in ScaBER cells and expressed in low amounts in RT4 cells. This evidence concerns the gene RACGAP1 and urothelial carcinoma.