In our study, ANLN expression was strongly correlated with cell proliferation markers including CDK1, RACGAP1, MKI67, and FOXM1. In urothelial carcinoma cell lines, these genes showed a consistently strong positive correlation with the expression of ANLN, which is highly expressed in ScaBER cells and expressed in low amounts in RT4 cells. The gene discussed is ANLN; the disease is urothelial carcinoma.