To further investigate whether the inhibition of cell growth and induction of apoptotic cell death of AML cells caused by combined treatment of SAHA and DAC was controlled by PI3K/AKT and Wnt/β-catenin signaling pathways, we assayed the protein expression levels of p-PI3K, p-AKT, Wnt1, p-GSK3, and p-β-catenin in U937 cells by western blot. The gene discussed is AKT1; the disease is acute myeloid leukemia.