Steady-state and IGF-II-mediated changes in intracellular transcript levels and secretion of TIMP1, TIMP4, and MMP3 were analyzed in NL, IPF, and SSc fibroblasts by real-time PCR (Fig 4A–4C) and ELISA (Fig 4D–4F). The gene discussed is TIMP4; the disease is idiopathic pulmonary fibrosis.