DSCC1 and colonic neoplasm: To investigate the effect of cytosolic DSCC1 on colon cancer cells, the DSCC1 mutant [the predicted nuclear-localization signal (NLS) of DSCC1 was mutated; DSCC1-NLSmut; Fig. S5A], expected to be located only in the cytosol, was prepared and compared with wild-type DSCC1.