VIM and non-small cell lung carcinoma: As shown in Figure 2C, in Transwell assays, cell migration and invasion were significant suppressed in both A549 and H226 cells after knockdown of SMAD2. Moreover, EMT was inhibited by knockdown of SMAD2 in the NSCLC cells, which was manifested as downregulated levels of p-SMAD2, N-cadherin, Vimentin, Snail, and matrix metalloproteinase 2 (MMP2), and upregulated E-cadherin, compared with their levels in the negative control cells (Figure 2D).