Although patients with Grade 3 tumours showed a tendency toward higher TIIC counts for both CD8 and PD-1 compared with patients with Grade 1 and Grade 2 tumours without a statistical significance regardless of its location, no significant difference was observed among the intratumoural locations in all the subgroups according to histological grade for both CD8 and PD-1 (Supplementary Fig. 4). This evidence concerns the gene CD8A and neoplasm.