Functional enrichment analysis showed that DE genes between tumorspheres treated with (+)-JQ1 and (−)-JQ1 were related to KEGG and Reactome pathways such as proteoglycans in cancer, pathways in cancer, MicroRNAs in cancer, extracellular matrix organization, degradation of the extracellular matrix and regulation of insulin-like growth factor (IGF) transport and uptake by insulin-like growth factor binding proteins (IGFBPs). This evidence concerns the gene IGF1 and cancer.