To that end, CD8 T cell recall responses were assessed with flow cytometry in cell cultures of the spleen (2*106 cells) and mediastinal lymph nodes (1*106 cells), which drain from the atherosclerosis prone aortic arch, incubated for 4 h with the ApoB100 derived peptides (10 μM/peptide) and Brefeldin A. The CD8 T cell percentage was significantly higher in the peptide-treated group compared to the PBS group and trended to be higher compared to the unpulsed DC/vehicle group in spleen (Fig. 2A) and blood (Supplementary Fig. S2) but not in mediastinal lymph nodes (Fig. 2D). This evidence concerns the gene CD8A and atherosclerosis.