MITF and Nematoda infectious disease: A nematode infection assay was carried out using two independent homozygous T3 transgenic RNAi lines (Mi-msp2 E1 and Mi-msp2 E2) expressing Mi-msp2 dsRNA; the number of galls, females and egg masses per gram root fresh weight were compared with those of control Arabidopsis plants (Fig. 3).