Double-staining using LYVE-1 and Ki67 antibodies confirmed that lymphatic endothelium in the area of subcapsular sinus in superficial CLNs were increased as early as 3 h after focal ischemia, and the lymphatic response in superficial CLNs was sustained until 24 h after focal ischemia (Fig. 1b–c). Here, LYVE1 is linked to ischemia.