LGR5+ cells, isolated from biopsies or cell lines, expressed higher levels of LSD1, which enhanced stemness by inducing the expression of key stemness genes, like SOX9, NANOG, LGR5, CD90, and CD24. On the other hand, LSD1 inhibition attenuated the expression of the aforementioned genes and the self-renewal and drug resistance potential of HCC-CSCs. Here, NANOG is linked to hepatocellular carcinoma.