Our research showed that IVM can cause a decrease in p-AKT (Ser473)/AKT and p-mTOR (S2448)/mTOR, which indicated that IVM inhibited AKT/mTOR signaling in U251 and C6 glioma cells, and the suppression of AKT/mTOR signaling may contribute to the activation of autophagy induced by IVM. This evidence concerns the gene AKT1 and central nervous system cancer.