CACYBP and hepatocellular carcinoma: To determine if cell cycle was affected by CACYBP expression in HCC cells, flow cytometry analysis was performed and the data revealed that CACYBP knockdown SK-Hep-1 and Huh7 cells displayed a significantly higher percentage of G1 phase cells and a lower percentage of S phase cells (Figure 5G and 5H), suggesting that CACYBP depletion caused typical cell cycle arrest at the G1/S phase in HCC cells.