LGR5 and hydrops fetalis: To verify the role of β-catenin in Pten loss induced SCC formation, we generated Lgr5-CreER;Ptenflox/flox;β-cateninflox/floxmice, whose Pten and β-catenin were deficient in Lgr5+ cells upon induction with tamoxifen (Lgr5-Pten-/--β-catenin-/- mice); we found that less severe HF and epidermal hyperplasia in the mice compared to Lgr5-Pten-/- mice (Figure 6A, C).