To further investigate the expression of AGER/RAGE and DGKK proteins in HCC tumour mice treated with high (4.0 g/kg/3d) and low-dose ascorbate (2.0 g/kg/3d), we performed immunohistochemical (IHC) staining of 18 tumour tissue samples (Figure 7) and observed weak membranous staining of AGER/RAGE in hepatoma cells and low-dose ascorbate tissue samples (Red arrow) (2.0 g/kg/3d IP), with the yellow arrow representing the area of ​​necrosis (Figure 7C-E). Here, AGER is linked to hepatocellular carcinoma.