KRAS and cancer: Noticeably, ascorbate could selectively kill KRAS mutant cancer cells by downregulating Glut1 expression to affect glucose consumption, but had no significant effect on the glycolytic rate in KRAS cells or BRAF Wild type (WT) cells 35, 36, similar to our results of ECAR in Huh-7 cells treated with different P-AscH- which have demonstrated that the glycolysis (Figure 4 Bi) and extracellular acidification rate (Figure 4 Bii) and were not significantly difference in ECAR (all p > 0.05).