Previous studies have shown there was a remarkably low K-Ras rate or no BRAF mutation in hepatocellular carcinoma cells 39, 40 and the mutations of KRAS and BRAF were not present in Huh-7 cells (http://amp.pharm.mssm.edu/Harmonizome/gene_set/HUH7/CCLE+Cell+Line+Gene+Mutation+Profiles), which might explain why glycolysis, as measured by our ECAR assay, was unaffected by P-AscH- in Huh-7 cells. This evidence concerns the gene KRAS and hepatocellular carcinoma.