Splenocytes from mice that had been intradermally injected with CpG-EVs displayed secretion of interferon-γ (IFN-γ) and cytotoxic T lymphocyte (CTL) activation upon ex vivo exposure to melanoma cells, while splenocytes from mice injected with CpG or a mixture of CpG and EVs did this to a much lesser extent 120. This evidence concerns the gene IFNG and melanoma.