Through tissue, cell and molecular levels analysis, we found that CREB1 was abnormally highly expressed in breast cancer and positively correlated with VASP expression, and the promoter region of VASP contained two CRE elements, and overexpression or activation of CREB1 could significantly promote the expression of VASP, which suggested that CREB1 could act as a transcription factor to activate the expression of VASP, thereby promoting the proliferation and migration of breast cancer cells. The gene discussed is CREB1; the disease is breast cancer.