Three to four weeks after infection of AAV-CaMKIIα-mito-GCaMP6f and AAV-hsyn-jRGECO1a in L2/3 of M1, we performed two-photon, dual-color imaging of [Ca2+]mito and [Ca2+]cyto in somas (L2/3) and dendrites (L1), through glass cranial window in awake, head-fixed mice (Fig. 2a–d, Supplementary Movies 1, 2). Here, RIC8B is linked to infection.