To further confirm the effect of lncRNA-HGBC on GBC tumorigenesis, we employed a complementary approach by developing cells to stably overexpress lncRNA-HGBC in GBC-SD and EH-GB1 cells that express low levels of endogenous lncRNA-HGBC (Fig. 2g and Additional file 3: Figure S2D). The gene discussed is GABBR1; the disease is Hemoglobin C Measurement.