Shuixian Du et al. investigated the effect of TM6SF2 E167K on the expression levels of TNF-α, IL-2, IL-6 and IL-8 in the HCC cell HEPA 1–6,and demonstrated that overexpression of the TM6SF2 E167K protein significantly up-regulates the expression of IL-2 and IL-6 [22]. This evidence concerns the gene TNF and hepatocellular carcinoma.