The objective of this study was to investigate the incidence and distribution of blp1 gene variants among clinical A. baumannii strains, belonging to the most globally spread clonal lineages, to assess the role of blp1 gene in virulence-related features and, most importantly, to evaluate the efficiency of Blp1 protein-based vaccine against A. baumannii in murine infection model. The gene discussed is TM2D2; the disease is infection.