In summary, we demonstrated that HCC cells co-treated with ASH and TRAIL will result in ROS production, DNA double strand breaks and p53-mediated PUMA and Bax up-regulation, followed by caspases activation and consequently inducing apoptosis in the HepG2 cell while sparing the normal hepatic LO2 cell. Here, TNFSF10 is linked to hepatocellular carcinoma.