Regarding the regulation of ALS markers, the association between the changes in EAA, BCAA, and leucine with the induction of autophagy (increase of the pSer555 ULK1/total ULK1 ratio) in the vastus lateralis but not in the deltoid muscles, may suggest that a large exercise volume can facilitate the anti-catabolic effect of amino acids to blunt the activation of autophagy. The gene discussed is ULK1; the disease is amyotrophic lateral sclerosis.