In addition, the percentages of CD3e+CD4+CD25+Foxp3+ cells in the spleens of mice with DSS-induced colitis that were treated with M2c macrophage exosomes were significantly increased (13.5 ± 3%; P < 0.001) compared with PBS-treated mice with DSS-induced colitis (9.3 ± 1%) (Figure 5, Table 5). This evidence concerns the gene CD4 and colitis.