To further confirm the tumor suppression function of Fbxw7 and Slc9a3, we transduced MEF-LoxP-KC cells with a different sgRNA targeting either Fbxw7 or Slc9a3. While MEF-LoxP-KC cells transduced with a different sgRNA targeting Slc9a3 consistently formed colonies in soft agar and tumors in nude mice allografts (Fig. 5B), we did not detect discernable colony formation in MEF-LoxP-KC cells transduced with a different sgRNA targeting Fbxw7. Thus, our results identify Slc9a3 as a potential tumor suppressor whose mutation in KrasG12D-expressing MEFs induces transformation. This evidence concerns the gene CALCA and neoplasm.