Furthermore, the western-blot was used to eluted the EGFR downstream signal pathway MAPK, the results demonstrated the Linc00976 significantly activated the MAPK pathway, with the phosphorylated ERK, MEK, P38, JUK, and target gene Cyclin D1, CDK2, MMP2, MMP9 expression upregulating, while inhibited in OTUD7B downregulated PC cells (Fig. 6f). The gene discussed is MMP9; the disease is pachyonychia congenita.