The western-blot assay was used to detected the G0/G1 phase regulating protein cyclin D1 and CDK2, the result revealed that cyclin D1 and CDK2 downregulated in Linc00976 knockdown PC cells, and upregulated in Linc00976 overexpressed PC cells (Fig. 2f). Here, CDK2 is linked to pachyonychia congenita.