For further analysis of the infection phenotype we performed confocal laser scanning microscopy (CLSM) in the tlr2+/+Tg (mpeg1:EGFP), tlr2+/−Tg (mpeg1:EGFP) and tlr2−/−Tg (mpeg1:EGFP) transgenic lines in which macrophages are fluorescently labelled (Fig. 4a, b and Additional file 4: Figure S4). This evidence concerns the gene MPEG1 and infection.