In this regard, as early as 5 days following 17D intratumoral treatment regimens (Fig 5A), the absolute number of CD8 T cells per gram of tumor was increased (Fig 5B), while CD4+FOXP3+ Tregs were markedly reduced in their numbers (Fig 5C), resulting in high CD8/Treg and conventional CD4 T (Tconv)/Treg ratios (Fig 5D). Here, CD4 is linked to neoplasm.