To further examine the effect of AQP4 on cell apoptosis, we performed a cell apoptosis assay, and the results indicated that silencing AQP4 significantly promoted the GBM cell apoptosis rates, via both immunofluorescence assay (Figure 4I, left panel) and Annexin V assay (Figure 4I, right panel). This evidence concerns the gene ANXA5 and glioblastoma.