ARID1A and neoplasm: When comparing the impact of the detected ARID1A mutations, one sample with a missense ARID1A p.(E1779G) mutation (which had been previously classified as VUS and predicted as benign using our in silico pipeline) showed a strong expression of ARID1A in 80% of the tumor nuclei, which is a similar extent of expression to that found in tissues with wild-type ARID1A (Fig. 2A).