Figure 3A shows the normalized read counts for these genes. A key point is that these are not necessarily all of the important genes that can differentiate between the sample types, nor are they necessarily the most differentially expressed. However, they are effective in discriminating between human CF sputum and in vitro samples when used together. Of note, although some of these genes are coregulated (ldh1 and lctP2; fur, sbnI, and sirA; and isdE and isdC), the performance of this gene set is not reliant on coregulated genes (Table S2). Here, FURIN is linked to cystic fibrosis.