SOD1 and amyotrophic lateral sclerosis: As shown in Fig. 5, the band intensity of the immunoprecipitated SOD1 (“Bound” fraction) was compared with that of recombinant SOD1 in a fixed amount; thereby, the CSF samples of the ALS cases (40 μL) were found to contain approximately 0.2 ng of the C4F6-reactive SOD1 as rough estimation, corresponding to the molar concentration of approximately 0.3 nM.