As observed in the ELISA results with C4F6 (Fig. 3a), ALS10 with the C111Y mutation in the SOD1 gene were characterized with relatively high apparent amounts of SOD1 reactive to apoSOD and EDI among the ALS cases, again implying the mutation-induced misfolding of SOD1 (Figs. 6a and b). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.