When a conformationally non-selective SOD1 antibody, FL-154, was used as a capture antibody for the sandwich ELISA, we found that apparent amounts of SOD1 in ALS were slightly fewer than those of non-ALS; however, the differences were less significant (P = 4.7 × 10− 2, Mann-Whitney U-test) (Fig. 3b). Here, SOD1 is linked to amyotrophic lateral sclerosis.