We found that SIRT4 silencing aggravated p65 nuclear translocation in HepG2 cells (Fig. 8e), whereas inhibition of NF-κB by its inhibitor ammonium pyrrolidinedithiocarbamate (PDTC) reversed the effects of shSirt4-Lv on MCP-1 cells (Fig. 8f), supporting our hypothesis that SIRT4 inhibited MCP-1 expression via the NF-κB pathway in HCC peritumour tissues. This evidence concerns the gene CCL2 and hepatocellular carcinoma.