Furthermore, vitamin D-inadequate HF diet significantly decreased muscle transcripts related to mitochondrial biogenesis such as PGC-1α, NRF1, and mitochondrial transcription factor A (Tfam) gene expression were by 50.7%, 70.9%, and 60.5% compared to the HF diet (Figure 4C). The gene discussed is NRF1; the disease is hydrops fetalis.