Significant elevation of CSF TDP‐43 and CSF NfL and plasma NfL levels in the ALS group compared with those in controls was preserved (P = 0.004 in Fig. S4A, P = 0002 in Fig. S4B, and P = 0.0156 in Fig. S4C, respectively), while the difference between the groups regarding CSF t‐tau did not reach significance (Fig. S4D). This evidence concerns the gene NEFL and amyotrophic lateral sclerosis.