RUNX2 and Global developmental delay: Analyses of transcription factors, such as sp7 and runx2, as well as extracellular matrix molecules, such as col10a1a, will provide insight into which steps of bone development are disrupted in au27. Given that the au27 mutation is lethal in EtOH, our current analyses cannot fully rule out a developmental delay nor can we determine whether later forming axial skeletal elements are disrupted.