Our genome-wide CRISPR screen successfully revealed a number of known causative genes and signaling pathways associated with EGFR TKI resistance, such as YAP signaling, PI3K-mTOR signaling and RAS-MAPK signaling pathways, reinforcing their significance in mediating mutant EGFR dependency in NSCLC. This evidence concerns the gene PIK3CA and non-small cell lung carcinoma.