Conversely, downregulation of BRF1 expression in PCa cells (with at least two out of three independent siRNAs) reduced proliferation (Figs. 1f, S2b and S3b; Table S7), with FACS analysis revealing reduced G1 subpopulation and increased G2 phase (Fig. 1g, h). Here, BRF1 is linked to posterior cortical atrophy.