Considering that infiltration of myeloid cells has been well-known as a poor prognostic factor in several tumor types [9, 10], multiple staining of H1HR, GPC3 [11–13] (marker of HCC), and CD68 [14, 15] (marker of myeloid cell) by IHC analysis was applied to explore whether H1HR is also expressed in myeloid cells and thus plays a role in the progression of HCC. Here, GPC3 is linked to neoplasm.